Coding

Part:BBa_K367003:Design

Designed by: Fernando Govantes   Group: iGEM10_UPO-Sevilla   (2010-06-29)


fecA coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1277
    Illegal BglII site found at 2190
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 76


Design Notes

Stop codon changed to TAATAA. PstI site at position 279 removed (CTGCAG changed to CTGCAA), as in BBa_J07018.


Source

Escherichia coli DH1, complete genome GenBank: CP001637.1

References